FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l01_n01_P1W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l01_n01_P1W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33022483
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG58600.029.94663815
CCGTACG59850.029.1005733
CGTCTGA62950.027.98459816
CGTAATC60600.027.21564338
CTCGTAT60850.026.07037744
TCGTATG64700.024.69245745
TCGGAAG76400.023.236723
CACGTCT78450.022.94201914
ATCGGAA77950.022.5438142
CACCGTA80900.021.33428631
ACCGTAC85100.021.2326832
CACACGT85300.020.91453612
GTAATCT83750.020.01565739
CAGTCAC90400.019.59043927
ACGTAAT87150.019.44018437
TACGTAA90050.019.33858536
AGTCACC95300.018.79582428
ACACGTC96500.018.60374313
TAATCTC95600.017.69982140
AATCTCG103350.016.35124641
Produced by FastQC (version 0.11.5)