FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l01_n01_P1W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l01_n01_P1W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24532896
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG263720.10749648145901732TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG46350.035.19786533
ACGTCTG52850.031.67238415
AGTCACG53800.030.4930428
CGTCTGA55650.030.28106916
TTCGGAA57150.028.54193536
ACGTTTC58500.028.19509132
TTTCGGA61000.026.8135235
CTCGTAT49000.026.77239844
GTCACGT61500.026.74768629
GTTTCGG62750.026.17393534
TCGGAAG65350.025.994933
CACACGT65350.025.7499312
ATCGGAA65350.025.7196962
TCGTATG51750.025.26274545
TCGGAAT65650.024.81251537
CACGTCT72000.023.49719614
ACACGTC78050.022.22293913
CACGTTT75750.021.95337731
GATCGGA81750.020.812491
CAGTCAC82050.020.3781127
Produced by FastQC (version 0.11.5)