FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l01_n01_P1W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l01_n01_P1W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31968805
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG355910.11133040474925478TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG67000.030.1602228
ACGTCTG69500.030.04120415
CGTCTGA73350.028.21919316
CACGTGG73300.027.99704731
TCGTATG64700.027.54428145
CTCGTAT67250.027.16912544
GTCACGT77900.025.93962529
ATCGGAA82050.025.3121412
TCGGAAG83350.025.0790923
CACGTCT86650.024.69151714
TATCTCG81750.023.61631441
ACGTGGC87800.023.55220232
CACACGT91400.023.13708512
GCCTTAT87400.023.09146337
TCACGTG93000.021.89712130
GGCCTTA97750.021.12968336
CTTATCT99350.020.33735839
CAGTCAC103450.020.25110627
ACACGTC106800.020.18000413
CCTTATC100450.020.0917838
Produced by FastQC (version 0.11.5)