FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l01_n01_P1W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l01_n01_P1W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32526988
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG54100.027.9048515
AGTCACG54600.026.95439728
TCGTATG56650.025.77895245
CGTCTGA59100.025.62020716
CGTGAAA63100.023.32227333
CTCGTAT56200.023.10262544
TGAAACG67800.021.86848835
CACGTGA68850.021.70184531
ACGTGAA68650.021.6987832
GTCACGT68500.021.64847829
TCGGAAG73550.020.8636443
AACGATC62450.020.716638
ATCGGAA73250.020.6726682
CACGTCT78750.019.5403314
AAACGAT76050.019.37836837
TCACGTG79100.018.9464130
CACACGT81950.018.91408312
GAAACGA78250.018.68960436
CAGTCAC91800.016.66922627
ACACGTC99400.016.38578213
Produced by FastQC (version 0.11.5)