FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l01_n01_P1W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l01_n01_P1W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35060001
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG47600.025.90723428
ACGTCTG49000.025.43731715
CGTCTGA51800.024.14933216
ACGTCCG54650.022.3991132
CTCGTAT55250.021.82935744
TCGTATG57150.020.90672945
TCGGAAG65700.019.110023
GTCCGCA64450.018.99078634
GTCACGT66250.018.85130929
ATCGGAA66550.018.6970522
CACGTCT71250.017.9349714
CCGCACA68450.017.65083536
CACACGT75250.016.9510912
TCACGTC75000.016.56186130
CAGTCAC81800.015.68069527
TCCGCAC88850.014.30708435
ACACGTC95000.014.0427513
CGCACAT86800.013.86762937
TCTCGTA98850.012.36037343
CGGAAGA108450.012.2201934
Produced by FastQC (version 0.11.5)