FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l01_n01_P1W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l01_n01_P1W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29647582
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCTCGTATG334260.11274443898999925TruSeq Adapter, Index 16 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG61750.030.7875915
CGTCTGA63100.030.27162716
TCGTATG60000.026.43945145
CTCGTAT61150.026.23691444
TCGGAAG74400.025.6454523
ATCGGAA75400.025.1861342
GTCCGCA75350.024.69378936
CACGTCT84350.022.80401614
CACACGT87050.022.25102612
ACCCGTC91250.020.91130632
CAGTCAC91250.020.78923827
CGCATCT87200.020.59138739
TCACCCG92050.020.53449230
TCCGCAT90600.020.31408137
ACACGTC100950.019.45465713
CCGTCCG105400.018.48605334
AGTCACC104250.018.45600128
CCCGTCC104000.018.28293833
CGTCCGC107650.018.22474135
GATCGGA107500.017.794741
Produced by FastQC (version 0.11.5)