FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l01_n01_P1W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l01_n01_P1W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28277940
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG60500.032.01830315
CGTCTGA64050.030.13840516
TCGGAAG75200.025.8817313
ATCGGAA76100.025.5167772
CTCGTAT52750.025.46645444
TCGTATG51700.025.15684745
CACGTCT81100.024.16167814
CACACGT83150.023.56557512
GTCAGAA88650.021.77577836
TGTCAGA92650.021.00522835
CAGTCAC92750.020.86531427
ACACGTC96600.020.7735113
AGTCACA95000.020.51351428
GTCACAT95650.019.92664729
AATCTCG88550.018.55036541
GATCGGA109250.017.9839651
CGGAAGA112100.017.7636724
ACATGTC112750.017.36324132
CCAGTCA112550.017.21275726
ATGTCAG116000.016.85501934
Produced by FastQC (version 0.11.5)