FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l01_n01_P1W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l01_n01_P1W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27690944
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCCG46800.028.31617734
CGTATCT46350.027.71869339
TCCGTAT47150.027.48609537
ACGTCTG49400.027.37135115
CGTCTGA51800.026.40744816
CCGTATC49450.026.07140738
CTCGTAT48650.024.97615244
TCGTATG48600.024.7699945
TTCCGTA58400.022.65324436
GTTCCGT62250.021.39638935
TCGGAAG64150.021.2204823
CACGTCT66600.020.80806414
ATCGGAA65950.020.6072982
CACACGT72150.019.05122812
TATCTCG68300.018.81188641
GTCACAG75750.017.76490829
CAGTCAC77950.017.66794227
AGTCACA82750.016.64321528
ACACGTC84750.016.61703513
TCACAGT83450.016.3143130
Produced by FastQC (version 0.11.5)