FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l01_n01_P1W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l01_n01_P1W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27221949
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG42650.027.06183615
CGTCTGA45250.025.40760216
TCGTATG45500.023.44108845
CTCGTAT46300.021.33527844
TCGGAAG57750.020.143393
ATCGGAA58350.019.7821982
CACGTCT61750.019.34627314
CACAGTC63250.018.14410831
CACACGT66950.018.0782812
AGTCAAC71700.016.25449834
ACACGTC79400.015.66859413
CAGTCAC74500.015.5555927
TCACAGT75150.015.24096230
GTCACAG75350.015.23051129
AATCTCG69150.015.09881941
AGTCACA78500.014.73439128
ACAGTCA85700.014.0996232
TCTCGTA75300.013.74604143
GATCGGA86250.013.6988591
CGGAAGA93150.012.9472034
Produced by FastQC (version 0.11.5)