FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l07n02_whole_embryo_12hpf_4.352000000a8a86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l07n02_whole_embryo_12hpf_4.352000000a8a86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49178559
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC1230620.2502350668713168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1258500.070.018971
AGCAGTG1423250.061.8621982
GCAGTGG1865050.047.2138143
CAGTGGT1869650.046.8392754
AGTGGTA1886000.046.4293635
TGGTATC1971300.044.2757157
GGTATCA1997150.043.8762938
GTGGTAT2019850.043.3643766
GTATCAA2079100.042.135099
AGAGTAC1958950.025.44767418-19
GCAGAGT2039800.024.55219316-17
TACATGG2064150.024.06314822-23
ACGCAGA2143450.023.72998214-15
AGTACAT2112000.023.62591620-21
CAACGCA2164900.023.50034712-13
CATGGGG1204250.023.07279424-25
ATCAACG2233400.022.77990210-11
CGCAGAG1966550.022.46345516-17
CAGAGTA1997100.021.85577818-19
GAGTACA2051050.021.23677820-21
Produced by FastQC (version 0.11.2)