FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l07n02_whole_embryo_12hpf_3.352000000a8a36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l07n02_whole_embryo_12hpf_3.352000000a8a36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45248589
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC1008550.2228909281568979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1089450.067.232771
AGCAGTG1190700.061.400692
GCAGTGG1592550.045.9468153
CAGTGGT1588450.045.866874
AGTGGTA1613600.045.1621135
GGTATCA1682800.043.256968
TGGTATC1697550.042.743977
GTGGTAT1718500.042.3694046
GTATCAA1721350.042.288129
AGAGTAC1617300.025.56604818-19
GCAGAGT1713500.024.28187416-17
TACATGG1704850.024.14437922-23
AGTACAT1747200.023.6692520-21
CAACGCA1812700.023.3581812-13
CATGGGG999300.023.10509724-25
ATCAACG1855150.022.79272710-11
CGCAGAG1628250.022.52823316-17
ACGCAGA1894950.022.37189314-15
CAGAGTA1654250.021.8713418-19
GAGTACA1692000.021.30889120-21
Produced by FastQC (version 0.11.2)