FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l07n02_whole_embryo_12hpf_2.352000000a8a0f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l07n02_whole_embryo_12hpf_2.352000000a8a0f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43818336
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC1093580.24957132101045554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1112050.069.5125961
AGCAGTG1259200.061.3725362
GCAGTGG1653350.046.730633
CAGTGGT1651200.046.5055354
AGTGGTA1680100.045.727045
TGGTATC1758000.043.4954157
GGTATCA1771500.043.2926758
GTGGTAT1799950.042.6031156
GTATCAA1838650.041.747469
AGAGTAC1727150.025.41238818-19
GCAGAGT1806900.024.43672216-17
TACATGG1823350.024.04411522-23
AGTACAT1870050.023.50086620-21
CAACGCA1913950.023.42516712-13
ACGCAGA1919500.023.36238514-15
CATGGGG1066050.022.9189624-25
ATCAACG1966050.022.82351710-11
CGCAGAG1732300.022.34149216-17
CAGAGTA1764350.021.64638518-19
GAGTACA1808800.021.0710220-21
Produced by FastQC (version 0.11.2)