FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l07n02_whole_embryo_12hpf_1.352000000a89e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l07n02_whole_embryo_12hpf_1.352000000a89e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53461268
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC1295810.24238295283231964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1351450.069.1444851
AGCAGTG1512350.061.7107282
GCAGTGG1992000.046.875713
CAGTGGT1979900.046.854714
AGTGGTA2013250.046.0871355
TGGTATC2103200.044.007657
GGTATCA2123900.043.692828
GTGGTAT2151550.043.1313136
GTATCAA2203150.042.140239
AGAGTAC2068550.025.4418218-19
GCAGAGT2166350.024.45808616-17
TACATGG2181500.024.05694622-23
AGTACAT2223300.023.71469720-21
CAACGCA2289650.023.53134512-13
ACGCAGA2316050.023.23440614-15
CATGGGG1278450.023.015724-25
ATCAACG2364200.022.79347410-11
CGCAGAG2077400.022.4930716-17
CAGAGTA2112300.021.80191418-19
GAGTACA2158700.021.28043220-21
Produced by FastQC (version 0.11.2)