FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l07n01_whole_embryo_12hpf_4.351000000a8a89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l07n01_whole_embryo_12hpf_4.351000000a8a89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49178559
Sequences flagged as poor quality0
Sequence length101
%GC22

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATC126900.076.356631
TGACTAG122300.075.498351
GCGCGAT133900.075.2395861
GACTAAC110450.075.122441
CCTTACG130850.074.560081
CGCTTCA119650.074.350561
GATCTAG127450.072.745911
CCACTAC136750.072.350991
CATATTC135550.072.009871
ATGCGAT123050.070.944491
CGTGAGC114900.070.930861
CTGATCC121200.069.988511
CCCATAC160250.069.718131
GCTGCAG138250.069.641071
GATCGCG116450.069.578651
CCGTACT123250.069.325641
ACGAGCC132700.069.223261
GACTGCG128700.069.085411
TGCGTGG136550.069.081211
GACCTGG116400.068.7920151
Produced by FastQC (version 0.11.2)