FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l07n01_whole_embryo_12hpf_3.351000000a8a39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l07n01_whole_embryo_12hpf_3.351000000a8a39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45248589
Sequences flagged as poor quality0
Sequence length101
%GC25

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC90500.076.6619641
TGACTAG102950.076.530371
CGCTATC113150.075.889081
CGCTTCA98550.075.271
CCTTACG109550.074.522331
GCGCGAT109750.073.433971
CCACTAC111350.071.9947051
GATCTAG110150.071.614211
CGTGAGC96800.071.574421
CTGATCC103200.071.2338261
GCTGCAG112350.071.0578161
ATGCGAT98150.070.686781
GATCGCG102550.070.202511
CCGTACT101800.069.879481
CATATTC113800.069.7348561
TGCGTGG113100.069.452191
GTAACTG110400.068.912481
CCCATAC139050.068.656971
CGATGGC120650.068.5720441
GAACCTA117950.068.20791
Produced by FastQC (version 0.11.2)