FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l07n01_whole_embryo_12hpf_2.351000000a8a02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l07n01_whole_embryo_12hpf_2.351000000a8a02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43818336
Sequences flagged as poor quality0
Sequence length101
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATC117150.076.747281
CGCTTCA101750.076.173461
TGACTAG111600.075.922641
GACTAAC90850.075.060441
CCTTACG114250.074.619161
GCGCGAT119750.073.930131
GATCTAG113050.073.309471
CCACTAC117750.072.76441
CATATTC121600.072.453651
GCTGCAG120000.071.083261
CTGATCC102700.070.841681
ATGCGAT100650.070.4432141
CCGTACT108800.070.320361
TGCGTGG119700.070.070421
CGTGAGC101250.069.884971
GACCTGG106900.069.880981
GATCGCG102400.069.285761
GTACTCC114300.069.264851
CGATGGC128600.069.101071
CCTACCA104150.069.079751
Produced by FastQC (version 0.11.2)