FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l07n01_whole_embryo_12hpf_1.351000000a89e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l07n01_whole_embryo_12hpf_1.351000000a89e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53461268
Sequences flagged as poor quality0
Sequence length101
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATC136400.077.866751
GACTAAC111750.076.374381
CGCTTCA127700.075.4685441
TGACTAG135200.075.324171
CCTTACG134300.075.2981951
GCGCGAT142450.074.959991
GATCTAG135200.073.988521
CGTGAGC123150.072.6229861
CCACTAC140050.071.494121
CCGTACT127850.070.696631
CCTACCA126150.070.368541
GCTGCAG142300.070.23011
TGCGTGG143350.069.914591
CCGGTAG124350.069.82031
CATATTC143900.069.7794651
ATGCGAT123350.069.5387651
CTGATCC127800.069.27411
ACGAGCC133700.068.9539641
GATCGCG124750.068.834561
GTACTCC137600.068.3810351
Produced by FastQC (version 0.11.2)