FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l06n02_trinidad_4.352000000a8aa0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l06n02_trinidad_4.352000000a8aa0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33378870
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG201250.016.5959345
GTATTGG244250.015.2082831
CCTATAC146150.014.8875363
TATACCG109500.014.5349285
TATACAG192500.014.4873715
GTATATA429200.014.2770361
CTATACT207600.014.1430374
GTATAGG147450.014.1425511
GTATAAG145950.013.8973451
TACACTG154800.013.6575025
TAGACTG106700.013.5360335
CCTAGAC101850.013.5267413
TAGACAG121550.013.2894245
TACACCG111350.013.2694345
TACACAG145150.013.2235045
GTCTAGG103750.012.8654881
GTATAGA170650.012.7765751
ACAGTGC161300.012.7536878
ACTGTGC147900.012.7206488
TCTATAC160700.012.6822873
Produced by FastQC (version 0.11.2)