FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l06n02_osat_95.352000000a8bff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l06n02_osat_95.352000000a8bff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53348752
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG336250.018.5662785
GTATATA787450.016.3238161
TTATACT446700.015.8049014
CTATACT347700.015.7138314
TAATACT460000.015.6681124
TATACAG318900.015.6134375
GTATTAT422550.015.4463381
GTATAAG248550.015.1748221
GTATAGG237000.014.8921481
GTATAGA297250.014.8300631
GTATAAT416150.014.7935351
GTATTAG277400.014.4870771
ATTATAC401600.014.2547863
TCTATAC281950.014.1201493
ATAATAC413800.013.8230333
TAGACTG172250.013.8187985
TATACCG166150.013.7542375
ACTGTGC235750.013.6637468
TACACTG254500.013.64650255
GTATTGG417850.013.5965161
Produced by FastQC (version 0.11.2)