FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l06n02_osat_61_1.352000000a8b1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l06n02_osat_61_1.352000000a8b1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8241196
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG57400.021.9336535
CTATACT80800.021.3438244
GTATAGG51500.020.753641
TACACTG39450.020.5933065
TAGACTG27750.020.2020765
CCTATAC57800.018.9039783
AGACTGT28550.018.6375526
ACTGTGC39150.018.445438
TGTACTG61100.018.2727385
TTATACT88350.017.852894
CTGTACT45450.017.5611274
GTACTGT64600.017.5033596
TACTGTG44250.017.5005327
TAATACT93550.017.3683784
ATACTGT60450.017.211746
TAGACAG28400.017.0631335
AGTACTG49250.016.8814145
TTACACT50700.016.8671424
ACACTGT50050.016.8014266
TAAGGCT31700.016.6356854
Produced by FastQC (version 0.11.2)