FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l06n02_osat_213.352000000a8c84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l06n02_osat_213.352000000a8c84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64552193
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG400950.017.086815
CTATACT409200.016.19664
GTATAGG304100.015.6049481
GTATATA868900.015.3019351
TATACAG388900.015.258455
GTATTGG545400.015.0414751
CCTATAC295600.014.5447473
ACTGTGC298750.014.4398888
GTATAAG281600.014.2708861
TAATACT474100.014.2700434
GTATAGA343750.014.24718951
GTATTAG298850.014.0670581
GTATTAT447500.013.9693461
TACACTG316900.013.8527385
TCTATAC316950.013.5500133
TAGACTG215000.013.5238045
TATACCG228150.013.3690475
TTATACT483650.013.3301154
GTATAAT445350.013.140821
TATTGGG576700.012.9821642