FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l06n01_osat_98.351000000a8c1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l06n01_osat_98.351000000a8c1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20674542
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG146400.024.1034155
GTATAGG127950.022.2665841
CTATACT194700.022.2295534
CCTATAC139850.021.2634543
TAATACT207750.019.7402944
TTATACT207550.019.4402524
ATACTGT148350.019.4181776
ACTGTGC101850.018.2519028
ATTATAC177350.017.7211153
TAGACTG71100.017.6967225
ATAATAC173250.017.4476243
TATACAG140850.017.1275065
TACACTG104100.017.0396775
TATAGGG128100.017.0376872
TCTATAC114350.016.986883
CCCTATA132050.016.9412422
TATACCG68050.016.4045645
GTATTAT182250.016.3811051
TATACTA276900.016.3481945
GTATAAG102250.016.3335841