FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l05n02_ogla_39.352000000a8d26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l05n02_ogla_39.352000000a8d26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18267916
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG181600.016.925751
GTATAGG74550.016.0587771
TATACTG112700.014.8391575
GTATAAG80100.014.649541
TATTGGG204300.014.4413492
GTATTAG85550.013.8273481
TACACTG83900.013.703895
GTATTAT124900.013.6549951
GTATATA238050.013.6305081
TGCGTTC127500.013.2261959
CCTAGAC63800.013.1809473
GGTGCGT147900.013.1043987
TATACCG57250.012.8631315
CTATACT114150.012.7777074
GTCTAGG71900.012.7522841
GTGCGTT140450.012.649588
GTATATG151750.012.5224941
TATACAG101650.012.4794195
TAGACTG56800.012.3795215
TCTATAC91900.012.3559583