FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l05n02_ogla_38.352000000a8cfe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l05n02_ogla_38.352000000a8cfe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14109796
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG89950.017.4306495
GTATAGG62300.015.7843871
GTATTGG123350.015.3666471
GTATAAG67250.014.9757621
TCTATAC72500.014.941463
CTATACT96750.014.2903494
GTATATA189600.014.15648651
TAGACAG51700.013.9686325
TATACCG45250.013.9647725
CCTATAC67150.013.8677573
TACACCG50750.013.8556365
GTATAGA75000.013.7449711
TATACAG82400.013.7230475
GTGTAGG60750.013.5283611
ACTGTGC65500.013.4898238
TTATACT104050.013.1964334
TACACTG64650.013.0078515
GTGTATG90500.012.913141
TAGACTG45200.012.8239615
TGCGTTC84750.012.7795149