FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l05n02_ogla_37.352000000a8c91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l05n02_ogla_37.352000000a8c91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21339596
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG137250.016.9623535
GTATAGG94600.015.3161621
GTATTGG194250.014.4777781
GTATAAG100650.014.3011221
TATACCG71750.014.1045755
ACTGTGC93950.013.3992718
GTATAGA123400.013.2814411
CTATACT139000.013.2623434
GTATATA298400.013.2613421
TACACCG72000.012.9997825
TACACAG93800.012.9669985
TATACAG124000.012.83587555
CCTATAC91550.012.818133
TAGACTG67800.012.6137775
TAGACAG79500.012.6100755
GTGTAGG91200.012.605551
TCTATAC114050.012.5388373
CCTAGAC69350.012.5369153
TACACTG99400.012.4754575
TGCGTTC128200.012.301759
Produced by FastQC (version 0.11.2)