FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l05n02_ogla_36.352000000a8c27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l05n02_ogla_36.352000000a8c27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17535705
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG109900.014.22310455
GTATTGG149350.012.9779251
GTATAGG74500.012.7533441
GTATAAG88500.012.5609641
GTATATA259900.011.84456351
TATACCG61000.011.5273125
GTACTAG124250.011.43207551
GTATAAT139950.011.4055621
GTATTAT143250.011.3749591
TACACTG74400.011.2392135
ATACTGT108250.011.1901366
TAGACTG54150.011.1429825
CCTATAC76100.011.1126883
TTATACT147700.011.0977454
GTATAGA101500.011.0457781
TATACAG107850.011.0132635
GTATTAG96300.011.0009171
CCTAGAC58650.010.7737633
GTATTAA135850.010.77063751
TACACCG52250.010.7298065