FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l05n02_ogla_35.352000000a8be2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l05n02_ogla_35.352000000a8be2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21310288
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG97300.016.942341
TATACTG134100.015.5188015
GTATAAG101800.015.3534111
GTATTGG182350.014.9020841
TACACTG92500.014.4336915
TATACCG71750.014.2373575
GTCTAGG72300.013.7986881
GTATATA294650.013.543461
CTATACT136350.013.2068094
TATACAG122350.013.0869595
GTGTAAG65700.012.8709751
AGTACCG55400.012.8645415
TAGACAG84250.012.8581295
GTGTATG132000.012.8484591
TCTATAC111900.012.73745253
TACACCG69100.012.6518095
ACAGTGC104850.012.5955718
GTGTAGG88400.012.4678761
TAGACTG69550.012.4333195
GTATAGA114800.012.3319321