FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l05n02_obar_24.352000000a8b0e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l05n02_obar_24.352000000a8b0e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24432231
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG152100.014.8690265
GTATTGG226900.014.739431
TATACCG83400.014.6410375
TACACTG111250.014.1362035
GTATAAG117600.014.0981031
GTATAGG106350.014.0706981
GTATATA334150.013.9608741
CCTATAC108400.013.6745553
CTATACT158150.013.3688984
TAGACTG75000.013.3667555
TACACCG76550.013.2823035
GTCTAGG81650.013.0908811
GTATTAG124400.012.9455911
ACTGTGC105750.012.8924688
GTATAGA138200.012.8560081
TAGACAG89150.012.7907115
TCTATAC128850.012.7579113
GTCTTAG74350.012.7149511
GGTGCGT174500.012.6859577
GTATATG230700.012.6639771
Produced by FastQC (version 0.11.2)