FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l05n01_ogla_39.351000000a8d29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l05n01_ogla_39.351000000a8d29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18267916
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG114700.016.5679025
GTATTGG176950.015.4439461
GTATAGG79200.015.0022311
TATACAG107400.014.6418075
CTATACT111750.014.0723174
GTATAAG79850.013.8682591
GTATTAG87450.013.8586631
TAGACTG57450.013.8101185
TATACCG55900.013.7681035
GTGTAGG81550.013.637441
GTATATA238750.013.4369711
TATTGGG197800.013.2584282
CCTAGAC59800.013.1883993
TACACCG65050.013.1460765
TACACTG86850.013.1284165
GTGTAAG56650.013.0038741
CCTATAC77300.012.9069913
GTATAGA100150.012.8130871
TACACAG81350.012.7896155
TCTATAC94650.012.7496513