FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l05n01_ogla_38.351000000a8cf1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l05n01_ogla_38.351000000a8cf1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14109796
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG94750.018.7530985
GTATTGG123300.016.3439641
TATACCG44200.016.0156525
GTATAAG63450.015.20614051
GTATAGG62250.014.7357621
TAGACTG47350.014.6491855
CCTATAC65950.014.5523323
TACACCG50000.014.4428935
GTATAGA78200.014.1613221
GTACCGT38650.014.0128576
CCTAGAC44150.013.9897093
GTATATA189200.013.9420431
TAGACAG53050.013.7916415
GTATTAG73600.013.7548671
ATACTGT94800.013.5309036
CTATACT94850.013.5245374
ACTGTGC64950.013.3828758
AGTACCG38700.013.3812335
TCTATAC69300.013.3689533
GTGTATG90250.013.271131