FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l05n01_ogla_37.351000000a8c94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l05n01_ogla_37.351000000a8c94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21339596
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG135900.015.9419375
TATACCG66750.015.5879355
GTATAGG97050.014.937581
GTATTGG188650.014.4368921
CCTATAC92850.013.7651963
GTATAGA121250.013.7594571
TAGACTG69850.013.7398085
TATACAG122250.013.7189725
GTCTAGG76200.013.5356991
GTATAAG99300.013.3067341
GTATATA301600.012.9543731
CTATACT138700.012.8459374
GTATTAG107600.012.81036951
ACTGTGC98450.012.7368148
TACACTG96100.012.7059015
CCTAGAC69650.012.4154443
TACACAG92400.012.3405635
TAGACAG81900.012.2403625
TCTATAC111100.012.1882913
GACTGTG58600.012.16070657
Produced by FastQC (version 0.11.2)