FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l05n01_ogla_35.351000000a8be5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l05n01_ogla_35.351000000a8be5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21310288
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG135700.016.2102495
GTATAGG93400.015.7754861
GTATTGG179100.015.0206521
GTATAAG101550.014.9774541
GTATAGA119850.014.7527451
GTGTAGG87900.014.32931
CCTAGAC72550.014.2765373
TATACCG69900.014.2735065
CCTATAC97400.013.4633743
GTATATA294000.013.4345041
TACACTG94700.013.294885
TAGACTG68450.013.048875
TATACAG125350.012.92464355
GTGTAGA92750.012.9138151
CTATACT136800.012.7810214
GTATTAG105600.012.6476641
TCTATAC115800.012.5139693
CCTACAC82050.012.3919313
GTGTAAG67950.012.3808871
ACAGTGC105750.012.3517568
Produced by FastQC (version 0.11.2)