FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l05n01_ogla_34.351000000a8b45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l05n01_ogla_34.351000000a8b45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39021839
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG245700.016.6294675
GTATAGG177850.016.4620291
GTATTGG347800.015.6743791
CCTATAC178750.015.6024463
TATACCG131950.014.726485
CCTAGAC129000.014.6954813
TATACAG221200.014.6911585
TACACTG181050.014.6164445
TACACCG124600.014.5275365
CTATACT247450.014.4003784
TAGACTG123350.014.3281075
GTATAAG181200.014.0330511
ACTGTGC169850.014.0104138
CTACACT179750.014.0089964
GTATAGA214700.013.7029831
TATTGGG380800.013.3250152
GTATATA538350.013.3223551
GTCTAGG141900.013.2973361
TACACAG172800.013.1972235
GTGTAGG164300.013.0754731