FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l05n01_obar_24.351000000a8b01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l05n01_obar_24.351000000a8b01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24432231
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG155350.016.1784615
GTATTGG228300.015.0727541
GTATAAG118650.014.7414441
GTATAGG112600.014.6892891
ACTGTGC102300.014.5327468
GTATATA346850.014.2100961
TACACCG82000.013.7317935
CTATACT158800.013.463874
TAGACTG80200.013.3883445
TACACTG109700.013.0795585
TATACCG80450.012.8152315
TTATACT198050.012.6188214
GTCTAGG83650.012.557011
CCTAGAC73350.012.3720613
TACACAG108500.012.3484415
TCTATAC130500.012.3423533
TATACAG143200.012.3422035
TATTGGG259600.012.1889382
TGCGTTC155400.012.1344069
CCTATAC107700.012.0876993
Produced by FastQC (version 0.11.2)