FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l04n02_obar_22.352000000a8cae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l04n02_obar_22.352000000a8cae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13408064
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG78600.015.2906885
GTATAAG58800.014.7857481
TATACCG41700.014.1258545
GTATTGG120350.013.7373411
GTATATA157000.013.6170311
GTATAGG58000.013.02382851
TACACCG43700.012.9358375
CTATACT76200.012.9045964
TACACAG54400.012.5745555
CCTAGAC45150.012.4153733
TCTATAC61450.012.2916663
TACACTG57950.012.2141115
ACTGTGC56400.012.1271568
CTACACT56750.012.0538464
TAATACT95500.012.037634
TATACAG74200.012.0360225
GTATTAT94850.011.770641
GTGTAGG57650.011.7019591
GTGTAAG38200.011.5661571
TAGACAG48600.011.5338635
Produced by FastQC (version 0.11.2)