FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l04n02_obar_21.352000000a8c41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l04n02_obar_21.352000000a8c41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17417538
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG107950.015.533925
GTATAGG76750.015.1658891
TACACCG56900.014.5266685
TATACCG60950.014.4187325
GTATTGG158550.014.2932931
GTATAAG82250.013.7474211
TATACAG97650.013.1347125
GTATATA238850.012.9688811
CTATACT111000.012.7104974
CCTATAC77250.012.6066413
GTATTAG90250.012.5814541
TTATACT134850.012.47042754
GTCTAGG60350.012.4382691
GTGTATG108750.012.3633831
TATTGGG173450.012.2153112
CCTACAC71850.012.0995253
GTATTAT131600.012.0578751
GTCTAAG50050.012.0553491
ACCGTGC75950.011.9450658
GTGTAAG53700.011.9437211
Produced by FastQC (version 0.11.2)