FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l04n02_obar_20.352000000a8bd5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l04n02_obar_20.352000000a8bd5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30965972
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG295400.015.1663321
TATACTG192050.014.8659445
TATACCG96300.013.9108985
GTATATA416200.013.3784211
CTATACT191200.013.3170884
GTATAGG134750.013.2567921
CCTATAC129900.013.1655513
TACACTG136350.013.1346525
TATTGGG328100.012.9733152
GTATTAG156850.012.8125391
TATACAG177750.012.5608955
GGTGCGT223700.012.5065097
GTATAAG142050.012.4417371
CTACACT131750.012.4394394
TGCGTTC196900.012.3510529
GTATAGA163550.012.3167091
GTGCGTT210600.012.2919078
GTATTAT223950.012.2194251
GTGTAGG130500.012.0866771
TACACCG98450.012.0147845
Produced by FastQC (version 0.11.2)