FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l04n02_obar_19.352000000a8b78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l04n02_obar_19.352000000a8b78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32046642
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG193700.015.25445
GTATAGG137100.015.1780661
GTATTGG293200.014.72921851
GTATATA410800.014.0978561
GTATAAG138850.013.8234131
CCTAGAC119150.013.4364493
GTATAGA171050.013.4154071
TAGACTG103050.013.32245355
CTATACT188700.012.9649174
TATACAG179600.012.8547715
CCTATAC134000.012.6564543
TACACTG149700.012.566315
TACACCG109700.012.5581525
GTGTAAG98750.012.46071
TATACCG101900.012.4471945
TATTGGG319900.012.4297542
GTATTAG159200.012.3846821
TCTATAC152750.012.2846983
GTGTAGG150500.012.18514251
ACTGTGC135450.012.1333488
Produced by FastQC (version 0.11.2)