FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l04n02_obar_17.352000000a8af0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l04n02_obar_17.352000000a8af0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18487479
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG110550.015.6409165
GTATTGG174300.015.4271021
GTCTAGG60400.014.2366221
CCTATAC80450.014.1125123
TACACTG83750.013.7829115
GTATAGG82250.013.631431
GTATAAG80300.013.5483141
TATTGGG200050.013.4641612
TACACCG59250.012.9881165
TATACCG58250.012.9664385
CCTAGAC56900.012.9405243
GTCTTAG59500.012.9349091
GTCCTAG65050.012.9268081
GTGCGTT127000.012.7534568
GTATTAG89150.012.5763891
ACTGTGC76500.012.4799218
GGTGCGT139150.012.4592077
CTATACA111750.012.242344
TATACAG100900.012.2405715
GTCTATG74550.012.235421
Produced by FastQC (version 0.11.2)