FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l04n02_obar_16.352000000a8a93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l04n02_obar_16.352000000a8a93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17312616
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG150600.015.2360151
TATACTG106150.014.81220155
GTATAAG82700.014.7056321
GTATAGG73100.014.2323291
TATACCG56450.013.7162475
GTCTAGG59450.013.4247651
GTGTAGG71650.013.127991
GTATATA225400.012.1399751
TAGACTG55500.012.0680685
TATTGGG169050.012.0549742
TAGACAG61250.011.8657985
CCTAGAC56850.011.7817633
TACACCG55250.011.6927925
TACACAG70900.011.5907495
TACACTG75150.011.4409275
GTATAGA93100.011.4300371
CTATACT109200.011.3969924
GGTGCGT113200.011.3290917
TCTATAC88200.011.3102543
TATACAG96350.011.2900375