FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l04n02_obar_15.352000000a8a43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l04n02_obar_15.352000000a8a43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25221302
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG210050.013.4127961
GTATAGG114250.013.3070281
TAGACTG75350.012.4829595
TATACTG157650.012.4147775
GTATATA366500.011.8872841
TAGACAG84000.011.8195955
GTATTAG134900.011.5517881
GTATAAG120850.011.4402011
CTATACT160350.011.3169674
GTCTAGG80700.011.1269351
GTGTAGG101700.011.071711
TATACCG79950.011.0517265
CCTATAC106950.010.8826943
GTACTAG169300.010.8322251
GTATAGA141700.010.8297881
ACTGTGC103400.010.4276778
GTATTAC108800.010.3928671
TCTATAC134200.010.3366893
TATTGGG244400.010.3022232
GTGTATG157350.010.23576451
Produced by FastQC (version 0.11.2)