FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l04n01_obar_22.351000000a8ca1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l04n01_obar_22.351000000a8ca1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13408064
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG80150.015.1730985
TATACCG43600.014.9269775
TACACCG46550.014.0830665
GTATAGA68600.013.8545071
GTATAGG59400.013.6802771
CTATACT81000.013.4306294
GTATTGG118300.013.2560591
GTGTAGG54050.013.0122161
TAATACT100950.012.89407354
GTATATA158650.012.8799321
TAGACTG38100.012.8425155
CCTATAC57850.012.8106753
GTATAAG57300.012.6059091
TATACAG74650.012.4091665
GTGTATG84950.012.3067831
ACTATAC64400.011.8027943
ACTGTGC56500.011.7705378
TAGACGG22300.011.7164385
TAGACAG47700.011.6521295
GTACCGT34400.011.4619236
Produced by FastQC (version 0.11.2)