FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l04n01_obar_21.351000000a8c44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l04n01_obar_21.351000000a8c44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17417538
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG110500.015.5622165
GTATTGG156000.015.1096031
TACACAG75950.013.6349595
TATACCG59150.013.4921035
GTATAGG78600.013.4222831
GTATATA241900.013.4177841
TACACCG58550.013.3869675
TAGACTG54350.013.2852325
GTATAAG82150.013.0736491
TCTATAC86550.012.9532513
CTATACT114850.012.9461974
TTATACT131800.012.8671294
TATACAG104450.012.779775
TATTGGG170900.012.7583862
ACTGTGC73850.012.4141158
TACACTG78200.012.2707115
CCTATAC76900.012.2313013
GTATTAG86300.012.2246981
TCTACAC78900.012.2222983
GTGTAGG71950.012.1529861