FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l04n01_obar_20.351000000a8bd8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l04n01_obar_20.351000000a8bd8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30965972
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG195300.015.5426395
GTATAGG128250.015.0810961
TATACCG98200.013.7866745
GTATAAG138800.013.6951381
TATACAG175000.013.3281425
TAGACTG96950.013.32745555
GTATTGG290050.013.3039021
TACACCG99450.013.1357255
GTATATA417250.012.9383181
GTGTAGG131250.012.8173961
TAGACAG110450.012.7737055
CTATACT192400.012.7402254
ACTGTGC131350.012.7298098
TTATACT236700.012.5433694
TCTATAC154450.012.3952073
TACACTG138850.012.17952255
GGACCGT64600.012.1332426
TACACAG131200.012.0931315
GTATTAT228400.011.9845651
GTGTATG200650.011.9130981