FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l04n01_obar_19.351000000a8b7b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l04n01_obar_19.351000000a8b7b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32046642
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG197600.016.1069645
GTATTGG292750.015.5511011
GTATAGG134500.015.01617051
TAGACTG101750.014.19269855
GTATAAG139600.013.9910061
TACACTG148600.013.8099075
CCTAGAC120000.013.776433
CTATACT199200.013.68851954
GTCTAGG138350.013.636531
TACACCG110500.013.5417285
TATACAG182050.013.3338645
TATTGGG323150.013.1861742
ACTGTGC136750.013.0607278
TAGACAG113600.013.04674155
GTATATA417800.013.034931
TATACCG99100.012.9424325
GTGTAGG139000.012.8548071
TCTATAC156750.012.6982883
TTATACT245200.012.68984
GTATAGA167900.012.5668031