FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l04n01_obar_17.351000000a8af3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l04n01_obar_17.351000000a8af3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18487479
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG112900.016.1154145
GTATTGG172600.015.5280571
CTATACT114900.014.6361124
ACTGTGC75500.014.4080118
GTATAGG82600.013.8648711
TAGACTG57300.013.5135565
GTCTAGG60900.013.49918651
GTATAAG84350.013.4645421
CCTATAC77350.013.2659493
TATACCG61450.013.1420685
TACACCG60150.013.1101935
TATTGGG202100.013.0924472
GTATATA245400.012.8966961
TCTATAC93050.012.7634623
TATACAG101000.012.6052095
GTGTAGG76650.012.5852741
CCTAGAC57000.012.584823
GTGTAGA84400.012.5557091
GTCCTAG66500.012.5053321
ACTATAC90600.012.374533
Produced by FastQC (version 0.11.2)