FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l04n01_obar_16.351000000a8a96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l04n01_obar_16.351000000a8a96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17312616
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG75400.015.8191541
TATACTG109500.015.1401995
GTATTGG158450.014.1556771
GTATAAG76650.013.8872661
GTCTAGG60050.013.2946581
TACACTG75400.013.1672545
TAGACTG54650.013.0383135
CTATACT107700.013.0117034
TATACCG54100.012.9074465
TCTATAC89600.012.4590923
CCTATAC76400.012.4354983
ACTGTGC78150.012.3388548
CCTAGAC57050.012.2401923
GTATAGA90950.011.9650271
CTACACT74750.011.883884
GTATTAT128500.011.8708771
TATTGGG177800.011.8623712
GTATATA230350.011.8620711
TATACAG96950.011.8573555
GTCTTAG56850.011.7860841
Produced by FastQC (version 0.11.2)