FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l04n01_obar_15.351000000a8a46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l04n01_obar_15.351000000a8a46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25221302
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG154050.014.5856915
GTATTGG208950.012.6453151
GTATAGG111950.012.5650691
TATACCG76500.012.3571775
GTATAAG121650.012.0319451
GTATATA362050.012.02331351
GTGTAGG98350.011.9349291
TACACCG77600.011.7534965
TACACTG106500.011.686365
TAGACTG75400.011.6554215
ACTGTGC103650.011.6407238
TATACAG139750.011.4212885
GTATTAG135500.011.363261
TCTATAC133600.011.2008413
GTATATG228600.011.0802161
CTATACT162400.010.9108824
GTATAGA140850.010.7966831
GTATTAT198550.010.7466641
CTACACT108000.010.7325554
GTGTAAG76600.010.6707991