FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l03n02_g9.352000000a8b5f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l03n02_g9.352000000a8b5f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31934844
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG168850.014.5745535
TAGACTG101350.014.5313015
GTATTGG250250.014.3525981
GTATATA359650.013.5006191
GTATAAG117500.013.1814311
TATACAG164500.013.0538635
GTATAGG120200.013.0039191
TATACCG100250.012.9847225
TACACCG119500.012.9205785
TAGACAG100400.012.3974995
TAATACT212450.012.3886194
GTATTAT195500.012.3452481
GTCTATA122150.012.1740111
CTATACT163150.012.1428014
GTAATAC133050.012.0691823
CTACACC187650.012.0511444
GTATAAT186700.011.9092481
ATAATAC191800.011.8648683
TTATACT197050.011.6932494
CCTACAC133800.011.6819623