FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l03n02_g7.352000000a8ae3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l03n02_g7.352000000a8ae3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59227649
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG433100.017.4417361
TAGACTG179950.016.8437295
GTCTATA213700.016.3404331
TACACCG245650.016.052065
GTATAGG206750.015.8326831
TATAGAC238900.015.6506643
TATACTG270750.015.5290265
GTGTAGG303650.014.7386741
GTATATA537600.014.6788211
GGTATAG233750.014.4307041
TACACAG259250.014.4036715
ACTGTGC251400.014.3794138
TATTGGG438400.014.3153412
CCTACAC270700.014.2157893
GTATAAG183250.014.0778611
AGACCTA270450.013.9115796
TATACAG270650.013.6741035
CTATACT254900.013.6431514
TATACCG179200.013.6268215
CTACACC376850.013.6153464